Diagnosis of resistant tuberculosis using photonics

Multidrug resistance is an increasing problem in infectious diseases. Combating them starts with rapid diagnostics.

The overall goal of FluoResYst is the development of a rapid multi-parameter detection system for multidrug resistance, exemplary for tuberculosis infections. Technology wise, a photonic platform is being developed to enable tuberculosis diagnostics outside a laboratory environment by means of a high degree of integration. The principle of the detection platform is based on time-resolved fluorescence measurements, which eliminates the need for complex optics and wavelength filters. This is made possible by the use of picosecond lasers and single photon avalanche diode (SPAD) arrays, whose switching times are optimized for the detection of short-lived fluorochromes. The platform will also be suitable for other multiparameter analyses in diagnostics, biotechnology and bioeconomics. The molecular biology-biochemistry approach consists of a novel detection method that uses fluorescence incorporation to literally light up the multi-resistance genes on a microarray. The combination of two innovations, the photonic integration of time-resolved fluorescence measurement for short-lived fluorochromes and the biochemical fluorescence-based assay, leads to a new detection technology that greatly simplifies complex analyses that were previously only accessible at great expense and thus makes them widely accessible.

Project partners:

  • LIONEX GmbH (consortium coordinator) ⇒ Webseite
  • IMMS Institut für Mikroelektronik- und Mechatronik-Systeme GmbH ⇒ Webseite
  • iC-Haus GmbH ⇒ Webseite
  • X-FAB ⇒ Webseite
  • DITABIS Digital Biomedical Imaging Systems AG ⇒ Webseite
  • Fraunhofer-Institut für Zelltherapie und Immunologie, Institutsteil Bioanalytik & Bioprozesse ⇒ Webseite
  • Microfluidic ChipShop GmbH ⇒ Webseite
  • Institut für Molekulare Diagnostik und Bioanalytik gGmbH

3rd FluoResYst Workshop


Second FluoResYst Workshop


Press Release: official Kick-off of the BMBF Project FluoResYst